Transfection

There are a number of reasons to way some transfections experiments fail and others succeed. Some examples that may have an impact on the results include plasma membrane composition, viability, proliferation rate and growth conditions. Finding the right type of transfection reagent is therefore difficult and if something works for commercial cell lines it offers little proof of functionality in primary cell lines.

Generally speaking, transfection reagents are either based on lipid or polymer formulations where each formulation type comes with its advantages and disadvantages. Together with our partner OZ Bioscience we offer reagents that combines lipid cationic formulation with polymers, a technique called TEE-Technology (Triggered Endosomal Escape) and where the main advantages with each reagent type is combined.

All of our transfections reagents can be combined with magnetic nanobeads, in a transfection technique called Magnetofection (more information here). In this method nucleic acids used in the transfection process are coupled with magnetic nanobeads. The complexes that are formed are coupled to the transfection reagent in the same way as in a normal transfection preparation. Directly after the complexes have been added to the cell plate it is placed over a magnet that pulls down the complexes down to the position of the cells. This dramatically increase the number of connection points between cells and transfection complexes leading to a much faster and efficient transfection process.

Nordic Diagnostica offers a full range of transfection products

• Improve any transfection method using CombiMag by harnessing the unique properties of Magnetofection
  
• Lullaby - Optimized Reagents for transfection with siRNA
  NeuroMag – Ideal solution for neuron transfection
• Transduction Enhancers
• Viro-MISCTTM – Method for simultaneous transduction and cell isolation