Human PAI-1 N-terminal cysteine, active fraction

Wild type PAI-1 cloned with a cysteine residue at the N-terminus patent pending. The active fraction is >95percent pure and >95percent active as determined by titration with HWM tc-urokinase and SDS PAGE. The protein is supplied in buffer with 1 mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate, 0.1M NaCl, 1mM EDTA, pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.

Storage temperature: -70 C
Transport temperature: Dry ice
Supplier: Molecular Innovations