Human Antithrombin III total antigen assay ELISA kit

Human Antithrombin III will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled polyclonal anti-human Antithrombin III primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of purified Antithrombin III and is measured along with the test samples. Color development is proportional to the concentration of Antithrombin III in the samples.

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Supplier: Molecular Innovations