Active mouse tPA functional assay ELISA kit

NOTE: This kit has been reformulated and is now sensitive enough to measure active tPA in normal plasma of mouse strains NSA/CF-1 9.9 ng/ml, C57BL6 1.4 ng/ml, and CD-1 0.4 ng/ml.

The sensitive measurement of functionally active mouse tPA is easily performed with this 96 well strip format ELISA kit. Active tPA will bind to biotinylated functionally active PAI-1 bound to the avidin coated microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. An HRP detection system using TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples.

The assay measures active tPA in the 0.05-50 ng/ml range.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody

Storage temperature: 4 C
Transport temperature: Cold pack
Supplier: Molecular Innovations