Active human PAI-1 functional assay ELISA kit

The sensitive measurement of functionally active PAI-1 is easily performed with this 96 well strip format ELISA kit. Functionally active PAI-1 present in plasma or culture media reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, anti human PAI-1 primary antibody binds to the PAI-1. Excess antibody is washed away, and bound antibody, which is proportional to the original active PAI-1 present in the plasma sample, is then reacted with the secondary antibody conjugated to HRP. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, uPA Plate, Secondary Antibody

NEW AND IMPROVED ASSAY! Sensitivity as low as 0.125 U/ml Standard curve linear to 25 U/ml Uses only 20 ul of sample Results in less than 2 hours Highly specific for only active PAI-1 Does not measure latent PAI-1 or PAI-1 in complex with tPA or uPA

Storage temperature: 4 C
Transport temperature: Cold pack
Supplier: Molecular Innovations