Protease Activity Assay Kit

Proteases are enzymes, naturally present in all living cells and tissues, involved in a multitude of physiological reactions that includes digestions of proteins in the food to highly regulated cascades. Proteases can either break specific peptide bonds, depending on the amino acid sequence of a protein or completely breakdown peptides to amino acids. In certain physiological conditions, protease activity can abolish a proteins function or an activation of a function. For studying proteins, tissues and cells are lysed, which release proteases in the sample and their activities can be measured using a protease assay. Cepham Life Sciences, protease assay kit is a sensitive, universal protease assay kit, which is designed for the quantitative determination of the proteases present in the protein sample and can detect approximately 10ng/ml of proteases in 2 to 24 hours incubation. The kit is supplied with a protease substrate: casein labeled with resorufin and with protease activities in the test sample, resorufin-labeled peptides are released, that cannot be precipitated by the precipitating agent. The concentration of these resorufin-labeled peptides in the supernatant is directly proportional to the proteolytic activity present in the test sample, which can be measured spectrophotometrically at 574nm.

Size: 1 unit
Transport temperature: Shipped with wet ice

Supplier: Bosterbio