Mouse tPA total antigen assay ELISA kit

NOTE: This kit has been reformulated and is now sensitive enough to measure total tPA in normal plasma of mouse strains NSA/CF-1 9.4 ng/ml, C57BL6 2.4 ng/ml, and CD-1 0.4 ng/ml.

The sensitive measurement of total mouse tPA antigen is easily performed with this 96 well strip format ELISA kit. Murine tPA binds to the capture antibody coated on the microtiter plate. Free, latent and complexed tPA will bind to the capture antibody. An HRP detection system using TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, tPA Antigen Capture Plate, Secondary Antibody

Storage temperature: 4 C
Transport temperature: Cold pack
Supplier: Molecular Innovations