C5a

Measurement of terminal complement pathway activation in experimental samplesRange: 0.1 - 1 ng/mlSensitivity:LOD: 0.01 ng/ml LLOQ: 0.050 ng/mlIncubation time: 2 hours 15 minutesSample volume: 20 µl dilute 1:20 for plasma 10 µl dilute 1:50 for serumSample type: Serum, EDTA- and citrated plasma.Sample preparation: The proper collection, storage and shipment of specimens are essential. Test immediately or stored up to 4 hours at 2-8 C or on ice. For longer-term storage the samples should kept frozen at -70 C with stabilizing solution Cat. No. A9576, dilute samples 1:1 with stabilizing solution.Reference values EDTA Plasma 0.37 74.33 ng/mlSerum 13.37 179.23 ng/mlSpecies: HumanSpecificity: C5a and C5a des-ArgTests: 96 testsMethod: ELISAIntended use:C5a is generated as a result of cleavage of the terminal complement protein C5,during activation of the complement system via the classical, alternative or lectin pathway. C5a is a low molecular weight approximately 9 kD protein fragment of 74 amino acids. C5 a is rapidly metabolized by the serum enzyme carboxypeptidase to more stable, less active, 73 amino acid form, C5a des-Arg.Research has associated elevated levels of fluid phase and adsorbed C5a with hemo-incompatibility of some biomaterials, particularly in extracorporeal circuits. Levels of C5a have also been associated with pathogenesis of a variety of disease states, including myocardial infarction, stroke, as well as vascular leak syndrome and associated kidney injury. The role of C5a in the pathogenesis of malaria and other infectious diseases, as well as sepsis, is likewise well documented.

Size: 96 Tests
Storage temperature: +2-8c
Transport temperature: RT

Delivery time: 2-14days

Supplier: TECO medical AG