Anhydrotrypsin

Treatment of trypsin with phenylmethylsulfonyl fluoride PMSF followedby a strong base leads to the conversion of Serine 195 to dehydroalanineat the active site 1. Alpha and beta forms of anhydrotrypsin areseparated by immobilized soybean trypsin inhibitor. The activity ofanhydrotrypsin is greatly reduced compared to native trypsin asquantified by chromogenic substrate 2. Anhydrotrypsin provided byour company is the single chain beta-form prepared from TypeXIII bovine trypsin.
References
1. Ako, H. et al. 1972 Biochem Biophys Res Commun. 47:1402-1407.
2. Olson, S.T. et al. 1995 J. Biol. Chem. 270:30007-30017.

Storage temperature: -70C
Transport temperature: Dry ice
Supplier: Molecular Innovations