Active porcine tPA functional assay ELISA kit

The sensitive measurement of functionally active porcine tPA is easily performed with this 96 well strip format ELISA kit. Active tPA will bind to biotinylated functionally active PAI-1 bound to the avidin coated microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. Inactive or complexed enzyme will not be detected. An HRP detection system using TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples.

Suggested additional reagents: 10X Wash Buffer, TMB Substrate, Avidin Plate, Secondary Antibody

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Supplier: Molecular Innovations